An Active Gelatin Coating Containing Eugenol and Vacuum Delays the Decay of Chinese Seabass (Lateolabrax maculatus) Fillets during Cold Storage: A Microbiome Perspective

The purpose of this study was to evaluate the effect an active gelatin coating containing eugenol and vacuum on microbial diversity Chinese seabass (Lateolabrax maculatus) during cold (−0.9 °C) storage. bacterial sequences in were observed using a high-throughput sequencing technique targeting V3–V4 region 16S Ribosomal DNA (rDNA) 0, 12th, 24th day, which showed more comprehensive estimate samples compared with enumeration. results revealed that species fresh rich, mainly including Carnobacterium, Glutamicibacter, Pseudomonas, abundance ratios 0.286, 0.160, 0.130, respectively. Pseudomonas Shewanella primary contaminants spoiled control samples, where increased from 0.220 0.174 12th day 0.802 0.163 Vacuum treatment could inhibit growth such when stored Brochothrix became superior genus. However, dominated storage until their 0.343 0.279, inhibition Carnobacterium gradually enhanced increasing concentrations eugenol. Furthermore, effective at inhibiting increase total volatile basic nitrogen. This confirmed reduce bacteria, reproduction main dominant spoilage delay seabass.